Greisman S E, DuBuy B
Proc Soc Exp Biol Med. 1975 Mar;148(3):675-8. doi: 10.3181/00379727-148-38608.
Healthy New Zealand rabbits were injected iv with an LD-80 dose of E. coli endotoxin. Twenty minutes later, after removal of over 50% of the endotoxin by the RES, exchange transfusion was performed, accomplishing a rapid and sustained reduction in the level of endotoxemia simulating that seen in animals rendered highly tolerant by seven prior sublethal injections of toxin. Depite such reduction in endotoxemia, 96-hr mortality was only slightly, and not significantly reduced compared to sham exchanged controls (70 vs 83% respectively). Additional control studies indicated that exchange tranfusion per se did not enhance endotoxin mortality. The findings directly support the concept that endotoxin tolerance is based primarily upon enhanced RES resistance to endotoxin toxicity rather than upon enhanced RES clearance of circulating endotoxin.
给健康的新西兰兔静脉注射致死剂量80%的大肠杆菌内毒素。20分钟后，在网状内皮系统（RES）清除超过50%的内毒素后，进行换血，使内毒素血症水平迅速且持续降低，模拟了经七次亚致死剂量毒素注射而产生高度耐受性的动物体内的情况。尽管内毒素血症有所降低，但与假换血对照组相比，96小时死亡率仅略有下降，且无显著差异（分别为70%和83%）。额外的对照研究表明，换血本身并不会增加内毒素导致的死亡率。这些发现直接支持了以下观点：内毒素耐受性主要基于网状内皮系统对内毒素毒性的抗性增强，而非基于其对循环内毒素清除能力的增强。
