Hou Y, Li Y, Tang J, Wu J, Zhang S, Zhang J, Zhu J, Prinz M
Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Aug;17(4):236-40.
To understand the allele structure and genetic polymorphism at eight STR loci in Chinese Han population and construct a preliminary database.
EDTA-blood specimens were collected from the unrelated individuals in Chengdu, China. The DNA samples were extracted with Chelex method and were amplified by PCR technique. The PCR products were analyzed by the PAG horizontal electrophoresis with discontinuous buffer system and by the approach of the automated fluorescence detection.
Five STR loci consist of simple repeat motifs, while three STR loci contain complex repeat structures. The STR polymorphisms at all of the eight loci have been observed in Chinese Han population. According to the obtained data, the combined exclusion probability and the combined discrimination power for these STR markers in Chinese population are 0.9978 and 0.999 999 992, respectively.
The obtained data are beneficial to understanding the population genetics of the eight STR loci in Chinese Han population. For forensic genetics, the obtained data can be used to calculate the probabilities dealing with the paternity test and the individual identification.
了解中国汉族人群8个STR基因座的等位基因结构和遗传多态性，并构建初步数据库。
采集中国成都无血缘关系个体的EDTA抗凝血标本。采用Chelex法提取DNA样本，并用PCR技术进行扩增。PCR产物采用不连续缓冲系统的PAG水平电泳及自动荧光检测方法进行分析。
5个STR基因座由简单重复基序组成，3个STR基因座包含复杂重复结构。在中国汉族人群中观察到了所有8个基因座的STR多态性。根据所得数据，这些STR标记在中国人群中的联合排除概率和联合鉴别力分别为0.9978和0.999999992。
所得数据有助于了解中国汉族人群8个STR基因座的群体遗传学。对于法医遗传学，所得数据可用于计算亲子鉴定和个体识别的概率。
