d'Herbomez M, Sapin R, Gasser F, Schlienger J L, Wémeau J L
Service de médecine nucléaire, hôpital Salengro, CHRU, 59037 Lille cedex.
Ann Biol Clin (Paris). 2000 Jul-Aug;58(4):445-51.
We compared eight antithyroid peroxidase antibody assay kits in two centres, by use of panel sera from 269 patients: controls (n = 100), patients with autoimmune thyroid diseases (n = 77 Graves' disease, Hashimoto's thyroiditis), with non autoimmune thyroid diseases (n = 69 nodular goiter, differentiated thyroid carcinoma), and with autoimmune disease without thyroid pathology (n = 23 diabetic subjects, rheumatoid polyarthritis). On the controls sera we observed different distributions of values. The cut-off values of each kit was, in most cases, similar to the value noted in the manufacturer's instructions. In the clinical study, we observed few differences. The majority of assays demonstrated high diagnostic performance. Some false positive results and the non assessment of some sera by competitive immunoassay were observed.
我们在两个中心使用来自269名患者的血清样本，对8种抗甲状腺过氧化物酶抗体检测试剂盒进行了比较：对照组（n = 100）、自身免疫性甲状腺疾病患者（n = 77例格雷夫斯病、桥本甲状腺炎）、非自身免疫性甲状腺疾病患者（n = 69例结节性甲状腺肿、分化型甲状腺癌）以及无甲状腺病变的自身免疫性疾病患者（n = 23例糖尿病患者、类风湿性多关节炎患者）。在对照血清中，我们观察到了不同的值分布。在大多数情况下，每种试剂盒的临界值与制造商说明书中注明的值相似。在临床研究中，我们观察到的差异较少。大多数检测方法显示出较高的诊断性能。观察到一些假阳性结果以及竞争性免疫分析对某些血清未作评估的情况。
