Hrabé de Angelis M H, Flaswinkel H, Fuchs H, Rathkolb B, Soewarto D, Marschall S, Heffner S, Pargent W, Wuensch K, Jung M, Reis A, Richter T, Alessandrini F, Jakob T, Fuchs E, Kolb H, Kremmer E, Schaeble K, Rollinski B, Roscher A, Peters C, Meitinger T, Strom T, Steckler T, Holsboer F, Klopstock T, Gekeler F, Schindewolf C, Jung T, Avraham K, Behrendt H, Ring J, Zimmer A, Schughart K, Pfeffer K, Wolf E, Balling R
Institute of Experimental Genetics, GSF Research Center for Environment and Health, Neuherberg, Germany.
Nat Genet. 2000 Aug;25(4):444-7. doi: 10.1038/78146.
In the post-genome era, the mouse will have a major role as a model system for functional genome analysis. This requires a large number of mutants similar to the collections available from other model organisms such as Drosophila melanogaster and Caenorhabditis elegans. Here we report on a systematic, genome-wide, mutagenesis screen in mice. As part of the German Human Genome Project, we have undertaken a large-scale ENU-mutagenesis screen for dominant mutations and a limited screen for recessive mutations. In screening over 14,000 mice for a large number of clinically relevant parameters, we recovered 182 mouse mutants for a variety of phenotypes. In addition, 247 variant mouse mutants are currently in genetic confirmation testing and will result in additional new mutant lines. This mutagenesis screen, along with the screen described in the accompanying paper, leads to a significant increase in the number of mouse models available to the scientific community. Our mutant lines are freely accessible to non-commercial users (for information, see http://www.gsf.de/ieg/groups/enu-mouse.html).
在后基因组时代，小鼠将作为功能基因组分析的模型系统发挥重要作用。这需要大量类似于从其他模式生物（例如果蝇和秀丽隐杆线虫）获得的突变体库。在此，我们报告一项在小鼠中的系统性全基因组诱变筛选。作为德国人类基因组计划的一部分，我们开展了大规模的ENU诱变筛选以寻找显性突变，并进行了有限的隐性突变筛选。在对超过14000只小鼠进行大量临床相关参数的筛选过程中，我们获得了182个具有各种表型的小鼠突变体。此外，目前有247个变异小鼠突变体正在进行基因确认测试，这将产生更多新的突变系。此次诱变筛选，连同随附论文中描述的筛选，使得科学界可获得的小鼠模型数量显著增加。我们的突变体系可供非商业用户免费使用（详情见http://www.gsf.de/ieg/groups/enu-mouse.html）。
