Bae S R
Department of Ophthalmology, Taejon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Seoul, Korea.
Korean J Ophthalmol. 2000 Jun;14(1):12-9. doi: 10.3341/kjo.2000.14.1.12.
In this study, we investigated whether retinal soluble proteins, such as S-antigen, play a role in the induction of nitric oxide by macrophages in vitro. Cells from the murine macrophage cell line RAW 264.7 and rat and rabbit peritoneal macrophages were incubated in the presence of retinal soluble protein. The nitrite level in the cultured supernatant was measured to determine nitric oxide production using the Griess reaction. S-antigen induced significant, dose-dependent nitrite production in both RAW 264.7 and rat peritoneal macrophages. The induction of inducible nitric oxide synthase by retinal protein was inhibited by the iNOS-specific inhibitor, aminoguanidine and the tyrosine inhibitor, genistein. These results show that soluble retinal protein significantly induces nitric acid production by macrophages. Increased production of reactive oxygen species by macrophages in the presence of this soluble retinal protein in vivo may accelerate photoreceptor degeneration in uveitis.
在本研究中，我们调查了视网膜可溶性蛋白（如S抗原）是否在体外巨噬细胞诱导一氧化氮的过程中发挥作用。将来自小鼠巨噬细胞系RAW 264.7以及大鼠和兔腹膜巨噬细胞的细胞在视网膜可溶性蛋白存在的情况下进行培养。使用格里斯反应测量培养上清液中的亚硝酸盐水平，以确定一氧化氮的产生。S抗原在RAW 264.7和大鼠腹膜巨噬细胞中均诱导出显著的、剂量依赖性的亚硝酸盐产生。视网膜蛋白对诱导型一氧化氮合酶的诱导作用被iNOS特异性抑制剂氨基胍和酪氨酸抑制剂染料木黄酮所抑制。这些结果表明，可溶性视网膜蛋白可显著诱导巨噬细胞产生硝酸。在体内存在这种可溶性视网膜蛋白的情况下，巨噬细胞活性氧生成增加可能会加速葡萄膜炎中光感受器的退化。
