Król W, Czuba Z P, Threadgill M D, Cunningham B D, Pietsz G
Department of Microbiology and Immunology, Silesian School of Medicine, Zabrze-Rokitnica, Poland.
Biochem Pharmacol. 1995 Sep 28;50(7):1031-5. doi: 10.1016/0006-2952(95)00237-t.
The effect of flavone (2-phenylbenzopyran-4-one) and three amino-substituted flavones on the production of nitrite by murine activated peritoneal macrophages was studied in vitro. Activated peritoneal macrophages obtained from mice pre-treated with concanavalin A (Con A) (in vivo), after exposure in vitro to lipopolysaccharide (LPS) at a concentration of 100 ng/ml, produced nitrite (20.3 +/- 2.5 nmol/10(6) cells), as measured after 24 hr by the Griess reaction. Stimulation of production of nitrite was inhibited by NG-monomethyl-L-arginine, suggesting that nitrite was formed via nitric oxide (NO.) as a product of metabolism of arginine. Stimulation was inhibited by flavone and the aminoflavones (20-100 microM). 3'-amino-4'-hydroxyflavone was the most potent inhibitor of nitrite production. Genistein (5,7-dihydroxy- 3-(4-hydroxy-phenyl)-4H-1-benzopyran-4-one) also inhibited production of nitrite, by a mechanism that appears not to involve protein tyrosine kinases. These results suggest that the flavones can modulate the immune responses and the inflammatory reactions by controlling production of nitric oxide.
体外研究了黄酮（2-苯基苯并吡喃-4-酮）和三种氨基取代黄酮对小鼠活化腹膜巨噬细胞亚硝酸盐产生的影响。从经伴刀豆球蛋白A（Con A）体内预处理的小鼠获得的活化腹膜巨噬细胞，在体外暴露于浓度为100 ng/ml的脂多糖（LPS）后，通过格里斯反应在24小时后测量，产生亚硝酸盐（20.3±2.5 nmol/10⁶个细胞）。NG-单甲基-L-精氨酸抑制亚硝酸盐的产生，表明亚硝酸盐是通过一氧化氮（NO·）作为精氨酸代谢产物形成的。黄酮和氨基黄酮（20 - 100 μM）抑制亚硝酸盐产生。3'-氨基-4'-羟基黄酮是最有效的亚硝酸盐产生抑制剂。染料木黄酮（5,7-二羟基-3-（4-羟基苯基）-4H-1-苯并吡喃-4-酮）也抑制亚硝酸盐产生，其机制似乎不涉及蛋白酪氨酸激酶。这些结果表明黄酮可通过控制一氧化氮的产生来调节免疫反应和炎症反应。
