Chen T H, Liao P H, Liao P Y, Guo H C
Kaohsiung J Med Sci. 2000 Apr;16(4):181-6.
CD8+ T cells recognize viral or tumor antigens of 8-10 residues derived from cytosolic proteins that are bound to the class I molecules of the major histocompatibility complex (MHC). To escape this immune surveillance, adenovirus expresses a protein, E3-19k, that specifically down-regulates the cell surface expression of class I MHC molecules on infected cells. To most effectively manipulate the T-cell response to virus-infected cells, it is essential to understand the mechanism by which viruses, such as adenoviruses, down-regulate the class I MHC function. We have subcloned the lumenal domain of adenovirus E3-19k protein in order to characterize its interactions with the class I MHC molecules. Several point mutations have also been generated on the E3-19k lumenal domain with either the first 96 or 108 amino acids. Attempts to crystallize the complexes between E3-19k and class I MHC molecule had been initiated.
CD8 + T细胞识别来源于细胞溶质蛋白的8 - 10个残基的病毒或肿瘤抗原，这些抗原与主要组织相容性复合体（MHC）的I类分子结合。为了逃避这种免疫监视，腺病毒表达一种蛋白质E3 - 19k，它特异性地下调感染细胞上I类MHC分子的细胞表面表达。为了最有效地操纵T细胞对病毒感染细胞的反应，了解诸如腺病毒等病毒下调I类MHC功能的机制至关重要。我们已经亚克隆了腺病毒E3 - 19k蛋白的腔结构域，以表征其与I类MHC分子的相互作用。还在E3 - 19k腔结构域的前96个或108个氨基酸上产生了几个点突变。已经开始尝试结晶E3 - 19k与I类MHC分子之间的复合物。
