Ishii H, Nakao K, Nishizaki K, Ichii S, Hatada T, Yamamura T
Second Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan.
Transpl Int. 2000;13(3):194-200. doi: 10.1007/s001470050686.
We investigated the long-term changes of the nitric oxide (NO)-related neural component after syngeneic total small bowel transplantation in rats. In the present study, the NO-related neural component was examined using the electrophysiological and NADPH-diaphorase histochemical technique. The rats were divided into four groups: an untreated young adult control group, an untreated 2-year-old control group, a group killed 1 month after transplantation, and a group killed 2 years after transplantation. A superfusion apparatus was used to evaluate the response of jejunal strips to electrical transmural stimulation. In the presence of adrenergic and cholinergic blockade, the inhibitory effect of L-N(G)-nitro arginine (L-NNA; a nitric oxide synthesis inhibitor) on nonadrenergic, noncholinergic (NANC) relaxation was expressed as a L-NNA-sensitive component. The L-NNA-sensitive component accounted for 41.6 +/- 4.6% (mean +/- SE), 43.1 +/- 3.5%,54.6 +/- 4.1%, and 55.8 +/- 3.5% in the young control group, 2-year control group, 1-month transplant group, and 2-year transplant group, respectively, being significantly higher in the transplant groups (p < 0.05). The actual strength of the L-NNA-sensitive component was 0.24 +/- 0.03 (mean +/- SE), 0.26 +/- 0.02, 0.44 +/- 0.04, and 0.46 +/- 0.04 mg of tension per mg of wet weight, respectively, also being significantly higher in the transplant groups (p < 0.001). In addition, the percentage of NADPH-diaphorase-positive fibers was 24.1 +/- 1.1% (mean +/- SE), 25.5 +/- 1.4%, 31.0 +/- 1.6%, and 30.9 +/- 2.0%, respectively, being significantly higher in the transplant groups (p < 0.05). These results suggest that NO neurons in the intrinsic jejunal nervous system have an adaptive role in maintaining intestinal graft motility.
我们研究了大鼠同基因全小肠移植后一氧化氮（NO）相关神经成分的长期变化。在本研究中，采用电生理和NADPH黄递酶组织化学技术检测NO相关神经成分。将大鼠分为四组：未处理的年轻成年对照组、未处理的2岁对照组、移植后1个月处死的组和移植后2年处死的组。使用灌注装置评估空肠条对经壁电刺激的反应。在肾上腺素能和胆碱能阻断的情况下，L-N（G）-硝基精氨酸（L-NNA；一种一氧化氮合成抑制剂）对非肾上腺素能、非胆碱能（NANC）舒张的抑制作用表示为L-NNA敏感成分。L-NNA敏感成分在年轻对照组、2岁对照组、1个月移植组和2年移植组中分别占41.6±4.6%（平均值±标准误）、43.1±3.5%、54.6±4.1%和55.8±3.5%，移植组显著更高（p<0.05）。L-NNA敏感成分的实际强度分别为每毫克湿重0.24±0.03（平均值±标准误）、0.26±0.02、0.44±0.04和0.46±0.04毫克张力，移植组也显著更高（p<0.001）。此外，NADPH黄递酶阳性纤维的百分比分别为24.1±1.1%（平均值±标准误）、25.5±1.4%、31.0±1.6%和30.9±2.0%，移植组显著更高（p<0.05）。这些结果表明，空肠固有神经系统中的NO神经元在维持肠道移植物运动方面具有适应性作用。
