Ishii H, Kusunoki M, Yamamura T, Utsunomiya J
Second Department of Surgery, Hyogo College of Medicine, Japan.
Eur Surg Res. 1997;29(5):362-7. doi: 10.1159/000129544.
We have previously shown that increased intestinal contraction after small bowel transplantation was associated with marked enhancement in the nonadrenergic, noncholinergic (NANC) intrinsic neural component. In the present study, we examined the effect of three NANC neurotransmitters (substance P, neurotensin, and cholecystokinin-octapeptide, CCK-OP) on jejunal longitudinal muscle strips after syngeneic total small bowel transplantation in rats. Fourteen rats were divided into a normal control group (n = 7) and a group tested 4 weeks after transplantation (n = 7). A superfusion system was used to evaluate the in vitro effects of the three neuropeptides in the presence of atropine and guanethidine. In the control group, maximum contractile response to substance P, neurotensin, and CCK-OP was 5.16 +/- 0.30, 1.46 +/- 0.15, and 1.13 +/- 0.17 mg tension/mg wet weight, respectively, while the values for the transplanted group were 5.71 +/- 0.29, 2.52 +/- 0.17, and 1.20 +/- 0.18 mg tension/mg wet weight. These results show that neurotensin-induced contraction increased significantly after transplantation, but substance P appeared to be the dominant NANC neurotransmitter in both normal and transplanted small bowel.
我们之前已经表明，小肠移植后肠道收缩增强与非肾上腺素能、非胆碱能（NANC）内在神经成分的显著增强有关。在本研究中，我们检测了三种NANC神经递质（P物质、神经降压素和八肽胆囊收缩素，CCK-OP）对大鼠同基因全小肠移植后空肠纵行肌条的影响。14只大鼠被分为正常对照组（n = 7）和移植后4周测试组（n = 7）。使用灌注系统在阿托品和胍乙啶存在的情况下评估这三种神经肽的体外作用。在对照组中，对P物质、神经降压素和CCK-OP的最大收缩反应分别为5.16±0.30、1.46±0.15和1.13±0.17mg张力/mg湿重，而移植组的值分别为5.71±0.29、2.52±0.17和1.20±0.18mg张力/mg湿重。这些结果表明，移植后神经降压素诱导的收缩显著增加，但P物质似乎是正常和移植小肠中主要的NANC神经递质。
