Vollmayr B, Keck S, Henn F A, Schloss P
Department of Biochemistry, Central Institute for Mental Health, 68159, Mannheim, Germany.
Neurosci Lett. 2000 Aug 25;290(2):109-12. doi: 10.1016/s0304-3940(00)01346-x.
In addition to elevated corticosterone levels, stress produces structural changes and neuronal damage especially in the hippocampus. In this line it has been shown, that in rats single or repeated immobilisation markedly reduces brain-derived neurotrophic factor (BDNF) mRNA levels in the hippocampal formation. Since this neurotrophin also controls the efficacy of serotonergic neurotransmission, the aim of the current study was to investigate the effect of acute immobilization stress on the expression of serotonin transporter (SERT) mRNA in the raphe nuclei as a parameter of serotonergic innervation. We have examined the expression of SERT mRNA and of BDNF mRNA in rats upon acute immobilisation by quantitative in situ hybridisation with a (35)S-labelled oligonucleotide probe. Elevated corticosterone levels in stressed animals confirmed as internal controls the effect of stress under our conditions. Acute stress led to a significant decrease of BDNF mRNA in the hippocampus and of SERT mRNA in the raphe pontis, but not in other raphe nuclei investigated. These data provide evidence for fast interactions between neurotrophins, corticosterone and serotonergic neurotransmission under stress conditions.
除了皮质酮水平升高外，应激还会产生结构变化和神经元损伤，尤其是在海马体中。在这方面，研究表明，在大鼠中，单次或反复固定会显著降低海马结构中脑源性神经营养因子（BDNF）的mRNA水平。由于这种神经营养因子还控制着血清素能神经传递的效率，因此本研究的目的是研究急性固定应激对中缝核中血清素转运体（SERT）mRNA表达的影响，以此作为血清素能神经支配的一个参数。我们通过用（35）S标记的寡核苷酸探针进行定量原位杂交，检测了急性固定后大鼠中SERT mRNA和BDNF mRNA的表达。应激动物体内皮质酮水平升高，作为内部对照证实了我们实验条件下应激的效果。急性应激导致海马体中BDNF mRNA和脑桥中缝核中SERT mRNA显著减少，但在所研究的其他中缝核中未出现这种情况。这些数据为应激条件下神经营养因子、皮质酮和血清素能神经传递之间的快速相互作用提供了证据。
