LaBuda C J, Fuchs P N
Department of Psychology, University of Texas at Arlington, PO Box 19528, Arlington, TX 76019, USA.
Neurosci Lett. 2000 Aug 25;290(2):137-40. doi: 10.1016/s0304-3940(00)01340-9.
A behavioral test paradigm that measures the aversive quality of stimulus-evoked pain in an animal model of neuropathic pain (L5 ligation) was tested for sensitivity to (1) different forces (476 and 202 mN) and frequencies (once every 15 or 30 s) of mechanical stimulation to the hyperalgesic paw and (2) different doses of the common antinociceptive compounds morphine (1 and 10 mg/kg) and gabapentin (30 and 90 mg/kg). Compared to non-ligated controls, the greater force (476 mN) and frequency (every 15 s) of mechanical stimulation of the hyperalgesic paw was associated with the greatest degree of escape/avoidance behavior. There was not a significant degree of escape/avoidance behavior at the lowest force (202 mN) and frequency (every 30 s) of mechanical stimulation. Compared to ligated vehicle treated controls, morphine (1 mg/kg) and gabapentin (90 mg/kg) decreased mechanical hyperalgesia and also attenuated the escape/avoidance behavior. The antinociceptive and antiaversive effects were found at doses that did not produce evidence of decreased motor activity. It is concluded that the behavioral test paradigm used to measure the aversiveness of stimulus-evoked nociceptive behavior is sensitive to different degrees of evoked pain and traditional analgesic compounds.
在神经性疼痛动物模型（L5结扎）中，一种用于测量刺激诱发疼痛厌恶性质的行为测试范式，被测试其对以下方面的敏感性：（1）对痛觉过敏爪进行机械刺激的不同力度（476和202毫牛顿）和频率（每15或30秒一次）；（2）常用抗伤害性化合物吗啡（1和10毫克/千克）和加巴喷丁（30和90毫克/千克）的不同剂量。与未结扎的对照组相比，对痛觉过敏爪进行更大力度（476毫牛顿）和频率（每15秒一次）的机械刺激，与最大程度的逃避/回避行为相关。在最低力度（202毫牛顿）和频率（每30秒一次）的机械刺激下，没有显著程度的逃避/回避行为。与结扎后给予赋形剂处理的对照组相比，吗啡（1毫克/千克）和加巴喷丁（90毫克/千克）可减轻机械性痛觉过敏，并减弱逃避/回避行为。在未产生运动活动减少证据的剂量下，发现了抗伤害性和抗厌恶作用。得出的结论是，用于测量刺激诱发伤害性感受行为厌恶程度的行为测试范式，对不同程度的诱发疼痛和传统镇痛化合物敏感。
