Sokawa Y, Sokawa J, Kaziro Y
Cell. 1975 May;5(1):69-74. doi: 10.1016/0092-8674(75)90093-8.
Under the balanced condition of growth of E. coli cells, no distinct difference is observed in stable RNA and protein synthesis between CP78 (rel+) and CP79 (rel minus), whereas a considerable difference is present in RNA accumulation between NF161 (rel+) and NF162 (rel minus), where NF161 smaller than NF162. The RNA content of NF161 is lower than that of NF162 in four different cultures with different growth rates. These two sets of isogenic pairs of rel+ and rel minus strains are commonly used in the study of rel gene function; however, NF161 is a mutant in the spoT gene whose product may be responsible for the degradation of ppGpp. The basal levels of ppGpp in these four strains growing with three different growth rates were examined: NF161 (rel+ spoT minus) has a much higher content of ppGpp than do other strains. Furthermore, the contents of ppGpp tend to be lower when the above four strains are growing at a faster rate. Thus a close correlation seems to exist between the content of RNA and the basal level of ppGpp under the condition of balanced growth.
在大肠杆菌细胞生长的平衡条件下，CP78（rel +）和CP79（rel -）之间在稳定RNA和蛋白质合成方面未观察到明显差异，而在NF161（rel +）和NF162（rel -）之间的RNA积累存在相当大的差异，其中NF161比NF162小。在四种不同生长速率的培养物中，NF161的RNA含量低于NF162。这两组rel +和rel -菌株的同基因对常用于rel基因功能的研究；然而，NF161是spoT基因的突变体，其产物可能负责ppGpp的降解。检测了这四种菌株在三种不同生长速率下生长时ppGpp的基础水平：NF161（rel + spoT -）的ppGpp含量比其他菌株高得多。此外，当上述四种菌株以较快速度生长时，ppGpp的含量往往较低。因此，在平衡生长条件下，RNA含量与ppGpp的基础水平之间似乎存在密切相关性。
