Ridell M, Baker R, Lind A, Ouchterlony O
Int Arch Allergy Appl Immunol. 1979;59(2):162-72. doi: 10.1159/000232255.
Ribosomal preparations consisting of crude ribosomes (CR), 30S subunits (30S) and 16S core particles (16S) from four strains of the species Mycobacterium bovis (BCG), Mycobacterium fortuitum, Mycobacterium phlei and Mycobacterium smegmatis were analyzed by immunodiffusion technique for taxonomical purposes. The ribosomal preparations tested contained several interspecies cross-reacting precipitinogens. The number of precipitinogens demonstrated at the homologous reactions was generally larger than the number of precipitinogens shown at the heterologous reactions indicating a probable presence of species-specific antigens in ribosomes. The largest number of possible species-specific precipitinogens was demonstrated when crude ribosomal preparations were studied. However, such precipitinogens were also shown in the 30S subunits and they were individually analyzed. The 16S core particles were dominated by cross-reacting precipitinogens. The number of ribosomal precipitinogens shared by M. phlei and M. smegmatis was large indicating a close taxonomical relationship between these two species. Apart from the four mycobacterial strains studied, 15 other strains representing the genera Mycobacterium, Arthrobacterium, Corynebacterium, Kurthia, Nocardia and Rhodococcus were included in the study. The presence of intergenerically cross-reacting precipitinogens in the ribosomal preparations was demonstrated.
为了进行分类学研究，采用免疫扩散技术分析了来自牛分枝杆菌（卡介苗）、偶然分枝杆菌、草分枝杆菌和耻垢分枝杆菌四个菌株的核糖体制剂，这些制剂包括粗核糖体（CR）、30S亚基（30S）和16S核心颗粒（16S）。所测试的核糖体制剂含有几种种间交叉反应沉淀原。同源反应中显示的沉淀原数量通常多于异源反应中显示的沉淀原数量，这表明核糖体中可能存在物种特异性抗原。研究粗核糖体制剂时，显示出的可能的物种特异性沉淀原数量最多。然而，在30S亚基中也显示出此类沉淀原，并对它们进行了单独分析。16S核心颗粒以交叉反应沉淀原为主。草分枝杆菌和耻垢分枝杆菌共有的核糖体沉淀原数量很多，表明这两个物种之间存在密切的分类学关系。除了所研究的四种分枝杆菌菌株外，该研究还包括代表分枝杆菌属、节杆菌属、棒状杆菌属、库特氏菌属、诺卡氏菌属和红球菌属的其他15个菌株。核糖体制剂中存在属间交叉反应沉淀原得到了证实。
