Baker R, Ridell M, Lind A, Ouchterlony O
Int Arch Allergy Appl Immunol. 1979;59(3):328-36. doi: 10.1159/000232277.
Various structures and other preparations from mycobacterial cells were analyzed by immunodiffusion. The preparations were obtained from four strains referred to the species Mycobacterium bovis (BCG), Mycobacterium fortuitum, Mycobacterium phlei and Mycobacterium smegmatis. They represented cell walls (CW), culture filtrates (CF), artificially disintegrated cell material (XP), protoplasms (PP), crude ribosomes (CR), ribosomal 50S subunits (50S), ribosomal 30S subunits (30S), ribosomal 16S core particles (16S) and ribosomal-free fractions (UCS). The preparations were analyzed by reference precipitation systems based on CF, CR and 30S preparations. Serological comparisons were made with material from the same species and cross-testing between material from different species was not performed. It was shown that from the precipitinogenic point of view the CF and XP materials were very similar and that they both contained protoplasmic material to a large extent. Furthermore, it was found that ribosomal precipitinogens constitute an important part of the precipitinogens in PP as well as in the CF and the XP preparations. The ribosomal precipitinogens were found to represent the two subunits as well as the 16S core particle. One ribosomal precipitinogen, designated beta, was shown in all preparations except the CW and the UCS.
通过免疫扩散分析了分枝杆菌细胞的各种结构和其他制剂。这些制剂取自四株菌，分别为牛分枝杆菌（卡介苗）、偶然分枝杆菌、草分枝杆菌和耻垢分枝杆菌。它们代表细胞壁（CW）、培养滤液（CF）、人工破碎的细胞物质（XP）、原生质（PP）、粗核糖体（CR）、核糖体50S亚基（50S）、核糖体30S亚基（30S）、核糖体16S核心颗粒（16S）和无核糖体组分（UCS）。基于CF、CR和30S制剂，通过参考沉淀系统对这些制剂进行了分析。采用来自同一物种的材料进行血清学比较，未对来自不同物种的材料进行交叉检测。结果表明，从沉淀原的角度来看，CF和XP材料非常相似，并且它们都在很大程度上含有原生质物质。此外，还发现核糖体沉淀原是PP以及CF和XP制剂中沉淀原的重要组成部分。核糖体沉淀原被发现代表两个亚基以及16S核心颗粒。除了CW和UCS外，在所有制剂中都显示出一种名为β的核糖体沉淀原。
