Rahaman M M, Huq I, Dey C R
J Infect Dis. 1975 Jun;131(6):700-3. doi: 10.1093/infdis/131.6.700.
The efficiency of MacConkey's agar in the isolation of various types of Shigella was compared with that of salmonella-shigella (SS) agar during an extensive 18-month outbreak of disease caused by Shigella. In all, 1,580 isolates of Shigella were obtained from 12,307 rectal swab and fecal samples of patients with diarrhea and their contacts by direct plating onto MacConkey's and SS agars. Shigella dysenteriae type 1 and Shigella flexneri constituted 55 percent and 33 percent of all isolates, respectively, with a smaller number of Shigella boydii and Shigella sonnei. MacConkey's agar was superior to SS agar in the detection of S. dysenteriae type 1; 83 percent of the isolates were detectable on MacConkey's agar, compared with 40 percent on SS agar. In contrast, 84 percent of S. flexneri isolates were detectable on SS agar, compared with 51 percent on MacConkey's agar alone. These findings confirm that, for the culture of fecal specimens thought to contain S. dysenteriae type 1, one of the media used should be noninhibitory. Shigella was isolated at a consistently and significantly higher rate from fecal samples than from rectal swabs.
在由志贺氏菌引起的长达18个月的大规模疾病暴发期间，比较了麦康凯琼脂和沙门氏菌-志贺氏菌（SS）琼脂分离各类志贺氏菌的效率。通过直接接种到麦康凯琼脂和SS琼脂上，从12307份腹泻患者及其接触者的直肠拭子和粪便样本中总共获得了1580株志贺氏菌分离株。1型痢疾志贺氏菌和福氏志贺氏菌分别占所有分离株的55%和33%，鲍氏志贺氏菌和宋内志贺氏菌的数量较少。在检测1型痢疾志贺氏菌方面，麦康凯琼脂优于SS琼脂；83%的分离株在麦康凯琼脂上可检测到，而在SS琼脂上为40%。相比之下，84%的福氏志贺氏菌分离株在SS琼脂上可检测到，而仅在麦康凯琼脂上为51%。这些发现证实，对于培养被认为含有1型痢疾志贺氏菌的粪便标本，所使用的培养基之一应是非抑制性的。从粪便样本中分离出志贺氏菌的比率始终且显著高于直肠拭子。
