通过透射电子显微镜和绿色荧光蛋白（GFP）表达揭示发育中小麦胚乳中含B型颗粒的淀粉体之间的突起和相互连接

B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression.

作者信息

Langeveld S M, van Wijk R, Stuurman N, Kijne J W, de Pater S

机构信息

TNO department of Plant Biotechnology, Center for Phytotechnology UL/TNO, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.

出版信息

J Exp Bot. 2000 Aug;51(349):1357-61.

PMID:10944148

Abstract

Starch granules in mature wheat endosperm show a bimodal size distribution. The formation of small starch granules in wheat endosperm cells was studied by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) after expression and targeting of fluorescent protein into amyloplasts. Both techniques demonstrated the presence of protrusions emanating from A-type granules-containing amyloplasts and the presence of B-type starch granules in these evaginations. Moreover, CLSM recordings demonstrated the interconnection of the amyloplasts by these protrusions, suggesting a possible role of these protrusions in interplastid communication.

摘要

成熟小麦胚乳中的淀粉粒呈现出双峰大小分布。通过将荧光蛋白表达并靶向到造粉体中，利用透射电子显微镜（TEM）和共聚焦激光扫描显微镜（CLSM）研究了小麦胚乳细胞中小淀粉粒的形成。两种技术均证实了含A型颗粒的造粉体发出突起，且这些突起中有B型淀粉粒存在。此外，CLSM记录显示这些突起使造粉体相互连接，表明这些突起在质体间通讯中可能发挥作用。