Chayen N E, Cianci M, Olczak A, Raftery J, Rizkallah P J, Zagalsky P F, Helliwell J R
Biological Structure and Function Section, Division of Biomedical Sciences, Imperial College School of Medicine, London SW7 2AZ, England.
Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):1064-6. doi: 10.1107/s0907444900008556.
The A1 subunit of the carotenoprotein alpha-crustacyanin, isolated from lobster carapace, has been crystallized using the vapour-diffusion method. The crystals, grown in solutions of ammonium sulfate containing methylpentanediol (MPD), diffracted to 2. 0 A. The crystals are stable to radiation. The space group of the crystals is P2(1)2(1)2(1). The unit-cell parameters are a = 41.9, b = 80.7, c = 110.8 A. 'Standard structure determination' has been unsuccessful within this crustacyanin family. Instead, an approach based on the S atoms is being undertaken involving softer X-rays at the SRS, Daresbury.
从龙虾壳中分离出的类胡萝卜素蛋白α-虾青蛋白的A1亚基，已采用气相扩散法进行了结晶。晶体在含有甲基戊二醇（MPD）的硫酸铵溶液中生长，衍射分辨率达到2.0 Å。这些晶体对辐射稳定。晶体的空间群为P2(1)2(1)2(1)。晶胞参数为a = 41.9、b = 80.7、c = 110.8 Å。在这个虾青蛋白家族中，“标准结构测定”尚未成功。取而代之的是正在采用一种基于硫原子的方法，该方法涉及在达累斯伯里的同步辐射光源（SRS）使用更柔和的X射线。
