Akaki T, Sato K, Shimizu T, Tomioka H
Department of Microbiology and Immunology, Shimane Medical University.
Kekkaku. 2000 Jul;75(7):477-82.
We examined profiles of intramacrophagial growth of M. tuberculosis (MTB) when mouse peritoneal macrophages (M phi s) were infected with the organisms at day 0 or day 7 after in vitro precultivation, and obtained the following results. First, the growth rate of the virulent MTB H37Rv strain as well as attenuated H37Ra strain was slower in M phi s which had been precultured for 7 days (M phi s [day 7]) than in freshly prepared M phi s without precultivation (M phi s [day 0]). The doubling time of MTB H37Rv was 2.2 and 2.9 days in M phi s [day 0] and M phi s [day 7], respectively, and that of MTB H37Ra was 2.9 and 3.6 days in M phi s [day 0] and M phi s [day 7], respectively. Second, MTB-mediated cytotoxicity in terms of the LDH release from infected M phi s was less marked in M phi s [day 7] than in M phi s [day 0], when they were infected with MTB of either the H37Rv or H37Ra strain. MTB H37Ra strain exhibited much weaker cytotoxic effects on host M phi s than did H37Rv strain. Third, when M phi s [day 7] were infected with MTB of either the H37Rv or H37Ra strain, they showed markedly lowered levels of reactive oxygen intermediate (ROI) production than did M phi s [day 0]. In contrast, the reactive nitrogen intermediate (RNI) producing ability of M phi s in response to MTB infection was not so markedly reduced in M phi s [day 7] from that of M phi s [day 0]. As mentioned above, the M phi s [day 7] did not permit accelerated growth of infected MTB, compared to the MTB growth in the M phi s [day 0]. It thus appears that ROI played a trivial role in the antimicrobial activity against MTB of murine peritoneal M phi s which had been precultured for long periods. Although it is regarded that RNI played more critical roles in M phi anti-MTB activity than did ROI, the present results also suggest that other kinds of antimicrobial effectors are required in M phi antimicrobial activity against MTB organisms, particularly in the case of M phi s after prolonged in vitro cultivation.
我们检测了在体外预培养0天或7天后用结核分枝杆菌（MTB）感染小鼠腹腔巨噬细胞（Mϕs）时，MTB在巨噬细胞内的生长情况，结果如下。首先，强毒株MTB H37Rv以及减毒株H37Ra在预培养7天的Mϕs（Mϕs [第7天]）中的生长速度比未预培养的新鲜制备的Mϕs（Mϕs [第0天]）慢。MTB H37Rv在Mϕs [第0天]和Mϕs [第7天]中的倍增时间分别为2.2天和2.9天，MTB H37Ra在Mϕs [第0天]和Mϕs [第7天]中的倍增时间分别为2.9天和3.6天。其次，当用H37Rv或H37Ra菌株的MTB感染时，就感染的Mϕs中乳酸脱氢酶释放而言，MTB介导的细胞毒性在Mϕs [第7天]中比在Mϕs [第0天]中不那么明显。MTB H37Ra菌株对宿主Mϕs的细胞毒性作用比H37Rv菌株弱得多。第三，当用H37Rv或H37Ra菌株的MTB感染Mϕs [第7天]时，它们产生的活性氧中间体（ROI）水平明显低于Mϕs [第0天]。相反，Mϕs对MTB感染产生反应性氮中间体（RNI）的能力在Mϕs [第7天]中与Mϕs [第0天]相比并没有明显降低。如上所述，与MTB在Mϕs [第0天]中的生长相比，Mϕs [第7天]不允许感染的MTB加速生长。因此，ROI在针对长期预培养的小鼠腹腔Mϕs中的MTB的抗菌活性中似乎起的作用微不足道。虽然人们认为RNI在Mϕ抗MTB活性中比ROI起更关键的作用，但目前的结果也表明，在Mϕ针对MTB生物体的抗菌活性中，特别是在体外长时间培养后的Mϕ情况下，还需要其他种类的抗菌效应分子。
