Shimuta K, Oda-Ueda N, Washio M, Oyama H, Oda K, Tsuru D
Department of Applied Microbial Technology, sojo University, Kumamoto, Japan.
Biosci Biotechnol Biochem. 2000 Jul;64(7):1542-6. doi: 10.1271/bbb.64.1542.
An expression and secretion system for scytalidopepsin B, an acid protease from Scytalidium lignicolum, was constructed in yeast. Saccharomyces cerevisiae AH22 was transformed with an yeast-E. coli shuttle vector, pAM82, in which an yeast invertase signal segment and the cDNA encoding the pro- and mature enzyme regions were inserted. The transformant was found to secret a pepstatin-insensitive acid protease, when cultured aerobically in a low phosphate (Pi) medium. Amino terminal amino acid sequencing analysis indicated that the recombinant acid protease was accurately processed and secreted as a mature form.
