Oda N, Gotoh Y, Oyama H, Murao S, Oda K, Tsuru D
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
Biosci Biotechnol Biochem. 1998 Aug;62(8):1637-9. doi: 10.1271/bbb.62.1637.
A chromosomal DNA of Scytalidium lignicolum was digested with Sau3AI. The digest was self-ligated and amplified by inverse PCR procedure using primers designed based on the nucleotide sequences of up- and down-stream regions of an intron present in the scytalidopepsin B gene. Analysis of the nucleotide sequence of PCR product (700 bp) showed that the enzyme is synthesized as a precursor protein consisting of the prepro- and mature enzyme regions. The deduced amino acid sequence was highly similar to those of aspergillopepsin A and recently reported endothiapepsins B and C, but quite different from those of pepstatin-insensitive bacterial acid proteases and the pepstatin-sensitive aspartic protease family.
用Sau3AI酶切木生弯孢霉（Scytalidium lignicolum）的染色体DNA。酶切产物进行自身环化，并通过反向PCR程序进行扩增，所用引物是根据弯孢霉胃蛋白酶B基因中一个内含子上下游区域的核苷酸序列设计的。对PCR产物（700 bp）的核苷酸序列分析表明，该酶以前体蛋白的形式合成，前体蛋白由前原酶区和成熟酶区组成。推导的氨基酸序列与曲霉胃蛋白酶A以及最近报道的内孢霉胃蛋白酶B和C的氨基酸序列高度相似，但与抑胃酶肽不敏感的细菌酸性蛋白酶和抑胃酶肽敏感的天冬氨酸蛋白酶家族的氨基酸序列有很大不同。
