A microfluidic cartridge to prepare spores for PCR analysis.
作者信息
Belgrader P, Okuzumi M, Pourahmadi F, Borkholder D A, Northrup M A
机构信息
Cepheid, Sunnyvale, CA 94089, USA.
出版信息
Biosens Bioelectron. 2000 Jan;14(10-11):849-52. doi: 10.1016/s0956-5663(99)00060-3.
Abstract
A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.
摘要
Zotero 插件