用于安全快速检测环境粉末和鼻拭子中炭疽芽孢杆菌孢子的新型样品制备方法。
Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs.
作者信息
Luna Vicki A, King Debra, Davis Carisa, Rycerz Tony, Ewert Matthew, Cannons Andrew, Amuso Philip, Cattani Jacqueline
机构信息
Center for Biological Defense, Department of Environmental and Occupational Health, College of Public Health, University of South Florida, Tampa, Florida 33612, USA.
出版信息
J Clin Microbiol. 2003 Mar;41(3):1252-5. doi: 10.1128/JCM.41.3.1252-1255.2003.
Abstract
Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.
摘要
炭疽芽孢杆菌孢子在美国曾被用作生物武器。我们希望开发一种安全、快速的样本制备方法，能为检测环境和临床样本中的孢子提供安全的DNA。我们的方法能可重复地检测出孢子含量低于10个的样本中的炭疽芽孢杆菌。
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