Looijesteijn P J, van Casteren W H, Tuinier R, Doeswijk-Voragen C H, Hugenholtz J
NIZO Food Research, Department of Flavour and Natural Ingredients, Ede, The Netherlands.
J Appl Microbiol. 2000 Jul;89(1):116-22. doi: 10.1046/j.1365-2672.2000.01082.x.
The type of substrate limitation had a remarkable influence on the molecular mass of exopolysaccharides (EPS) produced by Lactococcus lactis subsp. cremoris NIZO B40 and NIZO B891. Under glucose/energy limitation, the molecular mass was much smaller than under leucine or phosphate limitation, resulting in a marked decrease of the intrinsic viscosity of this EPS. The sugar composition of EPS produced by both strains, and the phosphate content of EPS produced by NIZO B40, were not affected by the type of nutrient limitation. Both strains produced comparable amounts of EPS under leucine and glucose limitation, but the efficiency of EPS production was highest under glucose limitation. The EPS yields of the phosphorylated B40 EPS as well as the unphosphorylated B891 EPS were reduced, with about 40% under conditions of phosphate limitation.
底物限制类型对乳酸乳球菌乳脂亚种NIZO B40和NIZO B891产生的胞外多糖（EPS）的分子量有显著影响。在葡萄糖/能量限制条件下，分子量比在亮氨酸或磷酸盐限制条件下小得多，导致该EPS的特性粘度显著降低。两种菌株产生的EPS的糖组成以及NIZO B40产生的EPS的磷酸盐含量不受营养限制类型的影响。在亮氨酸和葡萄糖限制条件下，两种菌株产生的EPS量相当，但在葡萄糖限制条件下EPS的生产效率最高。磷酸化的B40 EPS和未磷酸化的B891 EPS的产量均降低，在磷酸盐限制条件下约降低40%。
