Pandey S K, Werner L, Escobar-Gomez M, Werner L P, Apple D J
Center for Research on Ocular Therapeutics and Biodevices, Storm Eye Institute, Medical University of South Carolina, Charleston 29425-5536, USA.
J Cataract Refract Surg. 2000 Jul;26(7):1066-71. doi: 10.1016/s0886-3350(00)00297-2.
To evaluate the use of 2 dyes for staining the posterior capsule to enhance visualization during posterior continuous curvilinear capsulorhexis (PCCC).
Center for Research on Ocular Therapeutics and Biodevices, Storm Eye Institute, Medical University of South Carolina, Charleston, South Carolina, USA.
Indocyanine green 0.5% (ICG) and trypan blue 0.1% were used to stain the posterior capsule for performing PCCC in 8 human eyes obtained postmortem. The eyes were prepared according to the posterior video technique of Miyake-Apple. After CCC and hydrodissection/delineation were performed, the nucleus was carefully delivered by hydroexpression. Cortical cleanup was completed by irrigation/aspiration. The posterior capsule was stained by instilling 1 microdrop of ICG (4 eyes) or trypan blue (4 eyes) in the capsular bag. A PCCC was then performed by 2 independent surgeons (4 eyes/surgeon) not familiar with this technique. Each surgeon also performed PCCC in 2 eyes without the use of dye.
Both dyes facilitated the performance of PCCC after staining the otherwise transparent posterior capsule. It was easier to differentiate the stained posterior capsule flap from the underlying transparent anterior hyaloid phase of the vitreous. In addition, optic capture of an intraocular lens, with or without anterior vitrectomy, was easily accomplished because of the enhanced visualization by posterior capsule staining.
Posterior capsule staining can be successfully used to learn and perform the PCCC procedure, combined with optic capture, anterior vitrectomy, or both.
评估两种染料用于后囊染色以增强后连续环形撕囊术(PCCC)过程中的可视化效果。
美国南卡罗来纳州查尔斯顿市南卡罗来纳医科大学风暴眼研究所眼治疗与生物装置研究中心。
使用0.5%吲哚菁绿(ICG)和0.1%台盼蓝对8只死后获取的人眼后囊进行染色以实施PCCC。按照三宅 - 苹果后视频技术准备眼睛。在进行连续环形撕囊和水分离/划界后,通过水挤出小心娩出晶状体核。通过冲洗/抽吸完成皮质清理。在囊袋内滴入1微滴ICG(4只眼)或台盼蓝(4只眼)对后囊进行染色。然后由2名不熟悉该技术的独立外科医生(每位医生4只眼)进行PCCC。每位医生还在2只未使用染料的眼睛中进行PCCC。
两种染料在对原本透明的后囊进行染色后均有助于PCCC的实施。更容易将染色的后囊瓣与下方玻璃体的透明前玻璃体膜区分开来。此外,由于后囊染色增强了可视化效果,无论有无前部玻璃体切除术,人工晶状体的光学捕获都很容易完成。
后囊染色可成功用于学习和实施PCCC手术,结合光学捕获、前部玻璃体切除术或两者。