Jones J M, Feldman J D
Transplantation. 1975 Mar;19(3):219-25. doi: 10.1097/00007890-197503000-00004.
Soluble extracts were prepared from cells of Brown Norway (BN) rat spleens by sonication in 2 M KI. Extracts were fractionated by gel filtration and electrophoretic procedures. The fractions specifically inhibited the binding of Lewis alloantibodies to target BN cells in vitro and in vivo. A homogeneous component that retained antigen activity was obtained by preparatory polyacrylamide gel electrophoresis. Lewis rats injected with BN antigen fractions produced histocompatibility alloantisera that would prolong the survival of BN skin grafts applied to Lewis neonates.
通过在2M碘化钾中超声处理，从棕色挪威（BN）大鼠脾脏细胞中制备可溶性提取物。提取物通过凝胶过滤和电泳程序进行分级分离。这些级分在体外和体内均能特异性抑制Lewis同种抗体与靶BN细胞的结合。通过制备性聚丙烯酰胺凝胶电泳获得了具有抗原活性的单一成分。注射BN抗原级分的Lewis大鼠产生了组织相容性同种抗血清，该抗血清可延长应用于Lewis新生大鼠的BN皮肤移植物的存活时间。
