Cho J Y, Léveillé R, Kao R, Rousset B, Parlow A F, Burak W E, Mazzaferri E L, Jhiang S M
Department of Physiology and Cell Biology, Ohio State University, Columbus 43210-1218, USA.
J Clin Endocrinol Metab. 2000 Aug;85(8):2936-43. doi: 10.1210/jcem.85.8.6727.
The observation that radioiodide uptake (RAIU) activity, mediated by the Na+/I- symporter (NIS), is significantly increased in lactating breast suggests that RAIU and NIS expression in mammary gland are modulated by hormones involved in active lactation. We showed that both the NIS expression level and RAIU in rat mammary gland are maximal during active lactation compared to those in the mammary glands of virgin and pregnant rats as well as the involuting mammary gland. In the lactating mammary gland, NIS is clustered on the basolateral membrane of alveolar cells as a lesser glycosylated form than NIS in thyroid. The RAIU of lactating mammary gland was partially inhibited by treatment with a selective oxytocin antagonist or bromocriptine, an inhibitor of PRL release. These findings suggest that RAIU and NIS expression in mammary gland are at least in part modulated by oxytocin and PRL. Indeed, we showed that NIS messenger ribonucleic acid level was increased in a dose-dependent manner by oxytocin and PRL in histocultured human breast tumors.
由钠/碘同向转运体（NIS）介导的放射性碘摄取（RAIU）活性在泌乳期乳腺中显著增加，这一观察结果表明，乳腺中的RAIU和NIS表达受参与活跃泌乳的激素调节。我们发现，与未孕、怀孕大鼠以及退化期乳腺相比，大鼠乳腺中的NIS表达水平和RAIU在活跃泌乳期最高。在泌乳期乳腺中，NIS以比甲状腺中NIS糖基化程度更低的形式聚集在肺泡细胞的基底外侧膜上。用选择性催产素拮抗剂或溴隐亭（一种催乳素释放抑制剂）处理可部分抑制泌乳期乳腺的RAIU。这些发现表明，乳腺中的RAIU和NIS表达至少部分受催产素和催乳素调节。事实上，我们发现，在组织培养的人乳腺肿瘤中，催产素和催乳素可使NIS信使核糖核酸水平呈剂量依赖性增加。
