通过显微注射胎儿成纤维细胞核进行猪的克隆。

Pig cloning by microinjection of fetal fibroblast nuclei.

作者信息

Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H, Perry A C

机构信息

Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305-0901, Japan.

出版信息

Science. 2000 Aug 18;289(5482):1188-90. doi: 10.1126/science.289.5482.1188.

DOI:10.1126/science.289.5482.1188

PMID:10947985

Abstract

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.

摘要

猪的克隆将对肉类生产的优化和异种移植产生显著影响。为了从分化细胞克隆猪，我们将猪（野猪）胎儿成纤维细胞核显微注射到去核卵母细胞中，并通过电激活诱导发育。将110个克隆胚胎移植到4头代孕母猪体内，产下了一头外表正常的雌性仔猪。仔猪的毛色表明了其克隆来源，并通过DNA微卫星分析得到证实。