Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Faculty of Veterinary Medicine, Federal University of Santa Catarina, UFSC, Curitibanos, SC, Brazil.
Methods Mol Biol. 2023;2647:197-210. doi: 10.1007/978-1-0716-3064-8_10.
Somatic cell nuclear transfer (SCNT) has been successfully applied to clone animals of several species. Pigs are one of the main livestock species for food production and are also important for biomedical research due to their physiopathological similarities with humans. In the past 20 years, clones of several swine breeds have been produced for a variety of purposes, including biomedical and agricultural applications. In this chapter, we describe a protocol to produce cloned pigs by SCNT.
体细胞核移植（Somatic Cell Nuclear Transfer，SCNT）已成功应用于克隆多种动物。猪是主要的食用家畜之一，由于其与人类在生理病理上的相似性，在生物医药研究中也具有重要意义。在过去的 20 年中，已经为多种目的（包括生物医药和农业应用）生产了几种猪品种的克隆。在本章中，我们描述了一种通过 SCNT 生产克隆猪的方案。
