Byrne T J, Cohn G M, Lane M A, Adams L A, Hoffman D E, Reece M T
Department of Plant & Soil Sciences, University of Massachusetts, Amherst, MA, USA.
Oncol Rep. 2000 Sep-Oct;7(5):955-8. doi: 10.3892/or.7.5.955.
Previously we demonstrated that protein coded by the BRCA1 gene was expressed in normal human buccal cells. The present study confirmed that BRCA2 protein was similarly expressed in these cells. Messenger RNA for BRCA2 was detected with sequential use of two primer sets. Pooled cell samples from healthy donors reacted strongly with two commercially available antibodies, I17 and C15. Immunoreactivity was present in both cytoplasmic and nuclear compartments. We conclude buccal cells will provide a suitable model for exploration of normal BRCA function.
此前我们证明，BRCA1基因编码的蛋白质在正常人颊细胞中表达。本研究证实，BRCA2蛋白在这些细胞中也有类似表达。通过依次使用两组引物检测到了BRCA2的信使核糖核酸。来自健康供体的混合细胞样本与两种市售抗体I17和C15发生强烈反应。免疫反应性存在于细胞质和细胞核区室中。我们得出结论，颊细胞将为探索正常BRCA功能提供一个合适的模型。
