Aunoble B, Bernard-Gallon D, Bignon Y J
Laboratoire d'Oncologie Moleculaire, Centre Jean Perrin, BP 392, 63011 Clermont-Ferrand, France.
Oncol Rep. 2001 May-Jun;8(3):663-8.
Recent studies suggest that BRCA1 and BRCA2 expression, in response to cytotoxic agents, may be dependent on p53 status. To investigate this possibility, we quantified their transcripts in ovarian cancer cells, PA1 (wild-type p53), CaOV-3 (mutated p53) and SKOV-3 (null p53) exposed to four cytotoxic agents. In PA1, taxol and cisplatin had no effect, while adriamycin and ionising radiation (IR) induced both genes. In SKOV-3, expression decreased in response to taxol and cisplatin, and initially decreased then increased in response to adriamycin while IR had no effect. CaOV-3 responded similarly to SKOV-3, except for both genes being increased by cisplatin. We speculate that this regulation may be part of the survival response to certain cytotoxic agents and may be dependent in part on p53 status of cells.
近期研究表明，响应细胞毒性药物时，BRCA1和BRCA2的表达可能依赖于p53状态。为研究这种可能性，我们对暴露于四种细胞毒性药物的卵巢癌细胞PA1（野生型p53）、CaOV-3（突变型p53）和SKOV-3（p53缺失）中的它们的转录本进行了定量分析。在PA1细胞中，紫杉醇和顺铂没有影响，而阿霉素和电离辐射（IR）诱导了这两个基因的表达。在SKOV-3细胞中，响应紫杉醇和顺铂时表达下降，响应阿霉素时最初下降然后上升，而IR没有影响。CaOV-3细胞的反应与SKOV-3相似，只是顺铂使这两个基因的表达均增加。我们推测这种调控可能是对某些细胞毒性药物的生存反应的一部分，并且可能部分依赖于细胞的p53状态。
