Kaverin N V, Smirnov Y A, Govorkova E A, Rudneva I A, Gitelman A K, Lipatov A S, Varich N L, Yamnikova S S, Makarova N V, Webster R G, Lvov D K
The DI Ivanovsky Institute of Virology RAMS, Moscow, Russia.
Arch Virol. 2000;145(6):1059-66. doi: 10.1007/s007050070109.
In order to assess the degree of immune cross-protection among avian H2 influenza virus strains, mice were immunised with beta-propiolactone-inactivated virus preparations and infected intranasally with mouse-adapted variant of A/Black Duck/New Jersey/1580/78 (H2N3) strain. The experiments with 11 avian H2 strains revealed that both Eurasian and American H2 avian influenza viruses exhibit either high or moderate degree of cross-protection. The grouping of the strains in accordance with their cross-protection efficiency does not coincide with H2 phylogenetic branches. Several reassortant clones were obtained with the use of A/Pintail Duck/Primorie/695/76 (H2N3) strain and high-yield X-67 reassortant as parent viruses, among them a high-yield H2N3 reassortant. Taking into account the data on cross-protection among avian H2 strains, the high-yield H2N3 reassortant may be regarded as a prototype strain to be used for the preparation of killed vaccines in the case of a new appearance of avian H2 haemagglutinin in circulation in humans.
为评估禽H2流感病毒株之间的免疫交叉保护程度，用β-丙内酯灭活病毒制剂免疫小鼠，并经鼻内接种A/黑鸭/新泽西/1580/78（H2N3）株的小鼠适应变种。对11种禽H2株进行的实验表明，欧亚和美洲的H2禽流感病毒均表现出高度或中度的交叉保护。根据交叉保护效率对毒株进行的分组与H2系统发育分支不一致。以A/针尾鸭/滨海边疆区/695/76（H2N3）株和高产X-67重配株作为亲本病毒，获得了几个重配克隆，其中包括一个高产H2N3重配株。考虑到禽H2株之间交叉保护的数据，高产H2N3重配株可被视为一种原型毒株，用于在人类中出现新的禽H2血凝素时制备灭活疫苗。
