Schmitt R, Fausser J L, Lesot H, Ruch J V
INSERM U424, Institut de Biologie Médicale, Faculté de Médecine, Strasbourg, France.
Int J Dev Biol. 2000 Jun;44(4):403-8.
Hepatocyte growth factor (HGF) is considered to be one of the mediators of epithelio-mesenchymal interactions during early organogenesis and to be also involved in the development of murine molars. In the developing tooth, HGF is expressed in the cells of the dental papillae, and c-Met, its receptor, in the cells of dental epithelia. In order to study the functional role played by HGF in tooth development, we tested the effects of HGF translation arrest by anti-sense phosphorothioate oligodeoxynucleotides on E-14 molars cultured in vitro. We also analyzed the histo-morphogenesis and crown cytodifferentiation of transgenic met E-14 molars cultured in vitro. 3D reconstructions revealed perturbations of the cusp pattern. However, histo-morphogenesis and crown cytodifferentiation were normal at the histological level.
肝细胞生长因子（HGF）被认为是早期器官发生过程中上皮-间充质相互作用的介质之一，并且也参与小鼠磨牙的发育。在发育中的牙齿中，HGF在牙乳头细胞中表达，而其受体c-Met在牙上皮细胞中表达。为了研究HGF在牙齿发育中所起的功能作用，我们测试了反义硫代磷酸酯寡脱氧核苷酸阻止HGF翻译对体外培养的E-14磨牙的影响。我们还分析了体外培养的转基因met E-14磨牙的组织形态发生和牙冠细胞分化。三维重建显示牙尖模式受到干扰。然而，在组织学水平上，组织形态发生和牙冠细胞分化是正常的。
