Levy J G, Maier T, Kilburn D G
J Immunol. 1979 Mar;122(3):766-71.
Antigen-specific suppressor cells and suppressive extracts obtained from the thymuses of DBA/2 mice bearing small syngeneic P815 mastocytomas were compared for their immunogenetic properties and requirements. The assay for specific suppression involved the ability of either cells or extracts to inhibit the primary in vitro cytotoxic response of normal DBA/2 splenocytes to mitomycin-treated P815 cells. It was shown that pretreatment of suooressor cell populations with anti-Iad antiserum plus rabbit complement removed the suppressive activity. Similarly, absorption of the suppressor factor with anti-Iad antiserum removed the suppressive properties of the material. It was found that the suppressor cells, generated in DBA/2 tumor bearers, were capable of specifically suppressing the anti-P815 response of B6D2 F1 radiation chimeras possessing lymphoid cells of the H-2b or H-2t2 haplotype equally as well as they could suppress the response of H-2d-bearing effector cells. This indicates that the suppressor cells are not H-2 restricted with respect to K or D markers on the responder cells in this system.
对取自携带同基因小P815肥大细胞瘤的DBA/2小鼠胸腺的抗原特异性抑制细胞和抑制性提取物的免疫遗传特性及需求进行了比较。特异性抑制测定涉及细胞或提取物抑制正常DBA/2脾细胞对丝裂霉素处理的P815细胞的体外初次细胞毒性反应的能力。结果表明，用抗Iad抗血清加兔补体预处理抑制细胞群体可消除抑制活性。同样，用抗Iad抗血清吸收抑制因子可消除该物质的抑制特性。研究发现，DBA/2肿瘤携带者产生的抑制细胞能够特异性抑制具有H-2b或H-2t2单倍型淋巴细胞的B6D2 F1辐射嵌合体对P815的反应，其抑制效果与抑制携带H-2d的效应细胞的反应相同。这表明在该系统中，抑制细胞对于反应细胞上的K或D标记物不受H-2限制。
