Kilburn D G, Talbot F O, Levy J G
J Immunol. 1981 Dec;127(6):2465-9.
An antigen-specific helper factor (Hf) that enhances the in vitro generation of tumor-specific cytotoxic T cells (CL) has been isolated from the spleens of DBA/2 mice bearing the syngeneic mastocytoma, P815. This factor enhances the CL response to P815 but not to L1210 (a syngeneic T cell leukemia in DBA/2 mice), B10 minor antigens, or DBA/2 alloantigens. The factor binds specifically to P815 membrane immunoadsorbent columns and can be eluted with 2 M NaCl. P815 Hf did not resemble the lymphokine Il 2 because it did not enhance the proliferative or CL response of thymocytes nor would it support the growth of an IL 2-requiring line of T cells. This factor acted optimally if added late in the culture period in contrast to IL 2, which was more effective if added early. In limiting dilution experiments P815 Hf enhanced the level of CL activity generated but not the number of precursors triggered. IL 2 markedly amplified both of these parameters.
已从患有同基因肥大细胞瘤P815的DBA/2小鼠脾脏中分离出一种抗原特异性辅助因子（Hf），它可增强体外肿瘤特异性细胞毒性T细胞（CL）的生成。该因子增强了对P815的CL反应，但对L1210（DBA/2小鼠中的同基因T细胞白血病）、B10次要抗原或DBA/2同种异体抗原无增强作用。该因子特异性结合P815膜免疫吸附柱，可用2M NaCl洗脱。P815 Hf与淋巴因子IL-2不同，因为它既不增强胸腺细胞的增殖或CL反应，也不支持依赖IL-2的T细胞系的生长。与IL-2相反，如果在培养后期添加该因子，其作用最佳，而IL-2在早期添加更有效。在有限稀释实验中，P815 Hf提高了产生的CL活性水平，但未增加触发的前体细胞数量。IL-2显著放大了这两个参数。
