Affinity-labelling of Escherichia coli ribosomes by a derivative of phenylalanyl-tRNA. A critical test for the specificity.
作者信息
Bauer K, Czernilofsky A P, Kuechler E
出版信息
Biochim Biophys Acta. 1975 Jun 16;395(2):146-51. doi: 10.1016/0005-2787(75)90153-7.
PMID:1095073
Abstract
Under nonenzymatic binding conditions p-nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p-nitrophenoxycarbonyl-phenylalanyl-tRNA.
摘要
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