Schmitz C, Goebel I, Wagner S, Vomberg A, Klinner U
Lehr- und Forschungsgebiet Angewandte Mikrobiologie, Institute für Biologie IV (Mikrobiolgie), RWTH Aachen, Germany.
Appl Microbiol Biotechnol. 2000 Jul;54(1):126-32. doi: 10.1007/s002530000348.
An n-alkane-assimilating strain of Candida tropicalis was selected in sandy soil inoculated with microorganisms from contaminated sites. Competition experiments with n-alkane utilizers from different strain collections confirmed that yeasts overgrow bacteria in sandy soil. Acidification of the soil is one of the colonization factors useful for the yeasts. It can be counteracted by addition of bentonite, a clay mineral with high ion exchange capacity, but not, however, by kaolin. Strains of different yeast species showed different levels of competitiveness. Strains of Arxula adeninivorans, Candida maltosa, and Yarrowia lipolytica overgrew strains of C. tropicalis, C. shehatae or Pichia stipitis. Two strains of C. maltosa and Y. lipolytica coexisted during several serial transfers under microcosm conditions.
在接种了来自污染场地微生物的沙质土壤中筛选出了一株热带假丝酵母的正构烷烃同化菌株。与来自不同菌株库的正构烷烃利用菌进行的竞争实验证实，酵母在沙质土壤中比细菌生长得更快。土壤酸化是有利于酵母定殖的因素之一。添加具有高离子交换能力的粘土矿物膨润土可以抵消这种酸化作用，但高岭土则不能。不同酵母菌种的菌株表现出不同水平的竞争力。腺嘌呤营养型阿氏酵母、麦芽糖假丝酵母和解脂耶氏酵母的菌株比热带假丝酵母、谢氏假丝酵母或树干毕赤酵母的菌株生长得更快。在微观条件下进行的几次连续传代过程中，两株麦芽糖假丝酵母和解脂耶氏酵母共存。
