核酸与亮甲酚蓝反应的分子光谱研究
Molecular spectroscopy study of the reaction of nucleic acids with brilliant cresol blue.
作者信息
Wang Y T, Zhao F L, Li K A, Tong S Y
机构信息
Department of Chemistry, Peking University, Beijing, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2000 Aug;56A(9):1827-33. doi: 10.1016/s1386-1425(00)00260-2.
The interaction of brilliant cresol blue (BCB) with nucleic acids in aqueous solution has been studied by spectrophotometry and Rayleigh light scattering (RLS) spectroscopy. Under suitable conditions, the RLS spectra of BCB changed significantly due to the presence of nucleic acids. RLS intensity of BCB at 364 nm is greatly enhanced with the addition of nucleic acids, and a new RLS peak is observed at 552 nm. This peak is about half the intensity of that at 364 nm. The results of this study show that BCB interacts with DNA possibly due to the cooperative effect of electrostatic attraction, intercalation, coordination and hydrophobic effect. Under optimum conditions, the increase of RLS at 364 nm of a BCB solution is proportional to the concentration of nucleic acids added. This result is the basis for a new RLS method for determination of nucleic acids. The linear range of ctDNA, fsDNA and yRNA is 0.12-4.70, 0.11-4.64 and 0.43-7.07 microg ml(-1), respectively.
采用分光光度法和瑞利光散射（RLS）光谱法研究了灿烂甲酚蓝（BCB）与核酸在水溶液中的相互作用。在适宜条件下，由于核酸的存在，BCB的RLS光谱发生了显著变化。加入核酸后，BCB在364 nm处的RLS强度显著增强，并在552 nm处观察到一个新的RLS峰。该峰强度约为364 nm处峰强度的一半。研究结果表明，BCB与DNA相互作用可能是由于静电吸引、嵌入、配位和疏水作用的协同效应。在最佳条件下，BCB溶液在364 nm处RLS的增加与加入的核酸浓度成正比。这一结果是一种测定核酸的新RLS方法的基础。小牛胸腺DNA（ctDNA）、鱼精DNA（fsDNA）和酵母RNA（yRNA）的线性范围分别为0.12 - 4.70、0.11 - 4.64和0.43 - 7.07 μg ml⁻¹。
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