用共振光散射技术研究核酸与银纳米粒子 - 铝（III）的相互作用及其分析应用。
Study on the interaction of nucleic acids with silver nanoparticles--Al(III) by resonance light scattering technique and its analytical application.
作者信息
Zhou Haiping, Wu Xia, Yang Jinghe
机构信息
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Shanda Nanlu 27#, Jinan 250100, PR China.
出版信息
Talanta. 2009 May 15;78(3):809-13. doi: 10.1016/j.talanta.2008.12.054. Epub 2009 Jan 15.
It is found that Al(III) can further enhance the intensity of resonance light scattering (RLS) of the silver nanoparticles (AgNPs) and nucleic acids system. Based on this, a novel method of determination of nucleic acids is proposed in this paper. Under optimum conditions, there are linear relationships between the enhancing extent of RLS and the concentration of nucleic acids in the range of 1.0 x 10(-9)-1.0 x 10(-7) g mL(-1), 1.0 x 10(-7)-2.0 x 10(-6)g mL(-1) for fish sperm DNA (fsDNA), 1.0 x 10(-9)-7.0 x 10(-8)g mL(-1) for calf thymus DNA (ctDNA) and 1.0 x 10(-9)-1.0 x 10(-7)g mL(-1) for yeast RNA (yRNA). The detection limits (S/N=3) of fsDNA, ctDNA and yRNA are 4.1 x 10(-10)g mL(-1), 4.0 x 10(-10)gmL(-1) and 4.5 x 10(-10)g mL(-1), respectively. The studies indicate that the RLS enhancement effect should be ascribed to the formation of AgNPs-Al(III)-DNA aggregations through electrostatic attraction and adsorption bridging action of Al(III). And the sensitivity and stability of the AgNPs-fsDNA system could be enhanced by Al(III).
研究发现，Al(III) 可进一步增强银纳米颗粒（AgNPs）与核酸体系的共振光散射（RLS）强度。基于此，本文提出了一种测定核酸的新方法。在最佳条件下，对于鱼精DNA（fsDNA），RLS增强程度与核酸浓度在1.0×10⁻⁹ - 1.0×10⁻⁷ g mL⁻¹范围内呈线性关系，在1.0×10⁻⁷ - 2.0×10⁻⁶ g mL⁻¹范围内也呈线性关系；对于小牛胸腺DNA（ctDNA），在1.0×10⁻⁹ - 7.0×10⁻⁸ g mL⁻¹范围内呈线性关系；对于酵母RNA（yRNA），在1.0×10⁻⁹ - 1.0×10⁻⁷ g mL⁻¹范围内呈线性关系。fsDNA、ctDNA和yRNA的检测限（S/N = 3）分别为4.1×10⁻¹⁰ g mL⁻¹、4.0×10⁻¹⁰ g mL⁻¹和4.5×10⁻¹⁰ g mL⁻¹。研究表明，RLS增强效应应归因于通过Al(III)的静电吸引和吸附桥连作用形成了AgNPs - Al(III) - DNA聚集体。并且Al(III)可提高AgNPs - fsDNA体系的灵敏度和稳定性。
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