Collinder E, Berge G N, Grønvold B, Lindholm A, Midtved T, Norin E
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
Equine Vet J. 2000 Jul;32(4):345-50. doi: 10.2746/042516400777032165.
This study investigated the influence of zinc bacitracin on the intestinal flora of horses. The functionally active intestinal flora was examined in 6 horses during treatment with zinc bacitracin. Utilising gas chromatography, spectrophotometry, gel electrophoresis and paper chromatography, samples were analysed on biochemical markers reflecting the action of parts of the intestinal flora. The following 5 flora-related functions were studied in faecal samples and intestinal samples from different sections of the hindgut: conversion of cholesterol to coprostanol and of bilirubin to urobilinogens, degradation of mucin and of beta-aspartylglycine and inactivation of tryptic activity. Conversion to coprostanol, conversion to urobilinogens and degradation of mucin were affected by treatment of zinc bacitracin and conversion to coprostanol was most sensitive. All functions were normalised in a short time, in contrast to man and rats. Differences in environmental exposures are probably the reason for a more rapid normalisation of the intestinal flora functions in horses.
本研究调查了杆菌肽锌对马肠道菌群的影响。在6匹马使用杆菌肽锌治疗期间，对其功能活跃的肠道菌群进行了检测。利用气相色谱法、分光光度法、凝胶电泳法和纸色谱法，对反映肠道菌群部分作用的生化标志物样本进行了分析。对后肠不同部位的粪便样本和肠道样本研究了以下5种与菌群相关的功能：胆固醇转化为粪甾烷醇以及胆红素转化为尿胆原，粘蛋白和β-天冬氨酰甘氨酸的降解，以及胰蛋白酶活性的失活。杆菌肽锌治疗影响了粪甾烷醇的转化、尿胆原的转化和粘蛋白的降解，且粪甾烷醇的转化最为敏感。与人和大鼠不同，所有功能在短时间内恢复正常。环境暴露的差异可能是马肠道菌群功能更快恢复正常的原因。
