Wang X, Plomley J B, Newman R A, Cisneros A
PE-Sciex Instruments, Concord, Ontario, Canada.
Anal Chem. 2000 Aug 1;72(15):3547-52. doi: 10.1021/ac991425a.
An HPLC/MS/MS method has been developed for the characterization and quantification of the cardiac glycosides oleandrin, odoroside, neritaloside and the aglycone oleandrigenin, all contained in a patented-hot-water extract of Nerium oleander L (Anvirzel). Qualitative analysis of such extracts was achieved using a hybrid tandem quadrupole time-of-flight (QqTOF) mass spectrometer. Collision-induced dissociation (CID) mass spectra of oleandrin, oleandrigenin, odoroside, and neritaloside were obtained with greater than 5 ppm mass accuracy and resolution routinely in excess of 8000 (fwhm). The detection limit for oleandrin of 20 pg (injected) was realized when the precursor-to-product ion transition, m/z 577 --> 373, was monitored. We have also applied the analytical method to the determination of oleandrin, oleandrigenin, neritaloside, and odoroside in human plasma following an intramuscular injection of Anvirzel.
已开发出一种高效液相色谱/串联质谱（HPLC/MS/MS）方法，用于表征和定量夹竹桃（Nerium oleander L）专利热水提取物（Anvirzel）中所含的强心苷欧夹竹桃苷、奥多罗苷、夹竹桃苷和苷元欧夹竹桃苷元。使用混合串联四极杆飞行时间（QqTOF）质谱仪对这些提取物进行定性分析。欧夹竹桃苷、欧夹竹桃苷元、奥多罗苷和夹竹桃苷的碰撞诱导解离（CID）质谱常规获得的质量准确度大于5 ppm，分辨率超过8000（半高宽）。当监测前体离子到产物离子的跃迁m/z 577 --> 373时，实现了20 pg（进样量）欧夹竹桃苷的检测限。我们还将该分析方法应用于肌肉注射Anvirzel后人血浆中欧夹竹桃苷、欧夹竹桃苷元、夹竹桃苷和奥多罗苷的测定。
