肾上腺髓质素可抑制大鼠子宫的自发性收缩以及缓激肽诱导的收缩，但不能抑制催产素或前列腺素F（2α）诱导的周期性收缩。
Adrenomedullin inhibits spontaneous and bradykinin-induced but not oxytocin- or prostaglandin F(2alpha)-induced periodic contraction of rat uterus.
作者信息
Yanagita T, Yamamoto R, Sugano T, Kobayashi H, Uezono Y, Yokoo H, Shiraishi S, Minami S I, Wada A
机构信息
Department of Pharmacology, Miyazaki Medical College, Miyazaki 889-1692, Japan.
出版信息
Br J Pharmacol. 2000 Aug;130(8):1727-30. doi: 10.1038/sj.bjp.0703500.
In isolated rat uterine strips, adrenomedullin (AM) inhibited the spontaneous periodic contraction in a concentration-dependent manner (IC(50)=22.3+/-0.7 nM). The inhibitory effect of AM was prevented by either AM(22-52), a putative antagonist for AM receptors, or calcitonin gene-related peptide (CGRP)(8-37), a putative antagonist for CGRP receptors. AM also attenuated bradykinin (BK)-induced periodic uterine contraction, which was blocked by AM(22-52) or CGRP(8-37), whereas AM had no effect on the periodic contraction caused by oxytocin or prostaglandin F(2alpha) (PGF(2alpha)). RT-PCR analysis showed that mRNAs for calcitonin receptor-like receptor (CRLR), receptor-activity-modifying protein (RAMP)1, RAMP2 and RAMP3 were expressed in the rat uterus. These results demonstrate that AM selectively inhibits spontaneous and BK-induced periodic contraction via activating receptors for AM and CGRP.
在离体大鼠子宫肌条中，肾上腺髓质素（AM）以浓度依赖性方式抑制自发性周期性收缩（半数抑制浓度[IC(50)] = 22.3±0.7 nM）。AM的抑制作用可被AM受体的假定拮抗剂AM(22 - 52)或降钙素基因相关肽（CGRP）受体的假定拮抗剂CGRP(8 - 37)所阻断。AM还可减弱缓激肽（BK）诱导的子宫周期性收缩，该作用被AM(22 - 52)或CGRP(8 - 37)所阻断，而AM对催产素或前列腺素F2α（PGF2α）引起的周期性收缩无影响。逆转录-聚合酶链反应（RT-PCR）分析表明，大鼠子宫中表达降钙素受体样受体（CRLR）、受体活性修饰蛋白（RAMP）1、RAMP2和RAMP3的信使核糖核酸（mRNA）。这些结果表明，AM通过激活AM和CGRP的受体选择性抑制自发性和BK诱导的周期性收缩。
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