Patel M K, Gonzalez M I, Bramwell S, Pinnock R D, Lee K
Parke-Davis Neuroscience Research Centre, Cambridge University Forvie Site, Robinson Way, Cambridge, CB2 2QB.
Br J Pharmacol. 2000 Aug;130(8):1731-4. doi: 10.1038/sj.bjp.0703530.
In the present study we tested the effects of the antihyperalgesic compound gabapentin on dorsal horn neurones in adult spinal cord. Slices were taken from control and hyperalgesic animals suffering from streptozocin-induced diabetic neuropathy. At concentrations up to 100 microM, bath application failed to affect the resting membrane properties of dorsal horn neurones taken from both groups of animal. In contrast, bath application of gabapentin dramatically reduced the magnitude of the excitatory postsynaptic current (EPSC) in neurones taken from hyperalgesic animals without altering the magnitude of the EPSC in control animals. Using a paired pulse stimulation protocol, together with analysis of miniature EPSC's, it was possible to demonstrate that gabapentin mediated these effects via a pre-synaptic site of action.
在本研究中，我们测试了抗痛觉过敏化合物加巴喷丁对成年脊髓背角神经元的作用。切片取自对照组和患有链脲佐菌素诱导的糖尿病性神经病变的痛觉过敏动物。在浓度高达100微摩尔时，浸浴给药未能影响两组动物背角神经元的静息膜特性。相比之下，浸浴给予加巴喷丁可显著降低取自痛觉过敏动物的神经元中兴奋性突触后电流（EPSC）的幅度，而不改变对照组动物中EPSC的幅度。使用配对脉冲刺激方案并结合微小EPSC分析，可以证明加巴喷丁通过突触前作用位点介导这些效应。
