Xia R, Stangler T, Abramson J J
Physics Department, Portland State University, Portland, Oregon 97207, USA.
J Biol Chem. 2000 Nov 24;275(47):36556-61. doi: 10.1074/jbc.M007613200.
Hyperreactive sulfhydryl groups associated with the Ca(2+) release protein from sarcoplasmic reticulum are shown to have a well defined reduction potential that is sensitive to the cellular environment. Ca(2+) channel activators lower the redox potential of the ryanodine receptor, which favors the oxidation of thiols and the opening of the Ca(2+) release protein. In contrast, channel inhibitors increase the redox potential, which favors the reduction of disulfides and the closure of the release protein. Modulation of redox potential of reactive thiols may be a general control mechanism by which sarcoplasmic/endoplasmic reticulum, ryanodine receptors/IP(3) receptors, control cytoplasmic Ca(2+) concentrations.
与肌浆网钙释放蛋白相关的高反应性巯基具有明确的还原电位，该电位对细胞环境敏感。钙通道激活剂会降低兰尼碱受体的氧化还原电位，这有利于硫醇的氧化和钙释放蛋白的开放。相反，通道抑制剂会增加氧化还原电位，这有利于二硫键的还原和释放蛋白的关闭。反应性巯基氧化还原电位的调节可能是一种普遍的控制机制，通过该机制肌浆网/内质网、兰尼碱受体/三磷酸肌醇受体控制细胞质钙浓度。
