Vojvodić S
Zavod za transfuziju krvi, Novi Sad.
Med Pregl. 2000 Jan-Feb;53(1-2):7-14.
M and N blood group antigens demonstrate inhibitory activity in inhibition reaction of viral hemagglutination with some influenza virus strains, with help of N-acetylneuraminic acid (sialic acid) which occurs in glycophorins on the red blood cells surface, and represent specific hemagglutunation receptors and substrate for action of influenza virus neuraminidase.
Reactivity of human O red blood cells with MM and NN phenotypes is established in inhibition reaction of viral hemagglutination by influenza virus A2 Singapore, with intention to fortify possibility of using human red blood cells in viral hemagglutination, to determine their reactivity in titration, retitration of hemagglutinins and inhibition reaction of hemagglutination. The aim of investigation was to describe destinations between different red blood cells in view of speed of reaction and receptor capacity. Material included 69 samples of sera from persons infected with influenza virus, among them 32 samples were positive with titres 1/80 and more.
Reactivity of erythrocytes with MM and NN phenotypes in titration of hemagglutinins of influenza virus A2 Singapore in which base is viral hemagglutination is identical, because there are no statistically significant differences of average geomaterical levels of antibody titers. Enzymatically derived red cells by papain, which do not contain M and N blood group antigens, not cause viral hemagglutination phenomenon, because they sediment in all dilutions. Reactivity of red blood cells with MM and NN phenotypes in retitration of haemagglutinins and inhibition reaction of hemagglutination is identical, because there are no statistically significant differences in results with two kinds red blood cells.
Results of investigation revealed that the reactivity of O human red blood cells different in MN phenotype is identical in regard to speed of reaction and receptor capacity in titration, retitration and inhibition reaction of viral hemagglutinatination and also showed that they demonstrate viral hemagglutination phenomenon in contrast with papainised red blood cells which do not contain M and N blood group antigens, which indirectly means that M and N blood group antigens contain receptors for influenza virus.
Human red cells with MM and NN phenotypes cause viral hemagglutination phenomenon with influenza virus A2 Singapore, and could be used in routine virusological diagnostic procedures. O blood group red cells (MM and NN) in reaction of viral hemagglutination result identically in view of speed of reaction and receptor capacity, and have the same impact on result of this reaction. Enzymatically derived red cells by papain do not cause viral hemagglutination phenomenon, because they do not contain receptors for viral hemagglutinin on red cell membrane surface, which are hydrolazed by papain. Receptors for influenza virus on red cell membrane surface are a component part of M and N blood group antigens which are destroyed by papain.
M和N血型抗原在某些流感病毒株的病毒血凝抑制反应中表现出抑制活性，借助红细胞表面糖蛋白中存在的N-乙酰神经氨酸（唾液酸），并且代表流感病毒神经氨酸酶作用的特异性血凝受体和底物。
通过甲型流感病毒A2新加坡株的病毒血凝抑制反应确定人类O型红细胞与MM和NN表型的反应性，旨在增强在病毒血凝反应中使用人类红细胞的可能性，确定它们在血凝素滴定、再滴定以及血凝抑制反应中的反应性。研究目的是根据反应速度和受体能力描述不同红细胞之间的差异。材料包括69份感染流感病毒人员的血清样本，其中32份样本滴度为1/80及以上呈阳性。
在以病毒血凝为基础的甲型流感病毒A2新加坡株血凝素滴定中，MM和NN表型红细胞的反应性相同，因为抗体滴度的平均几何水平无统计学显著差异。经木瓜蛋白酶处理后不含M和N血型抗原的红细胞不会引起病毒血凝现象，因为它们在所有稀释度下都会沉淀。MM和NN表型红细胞在血凝素再滴定和血凝抑制反应中的反应性相同，因为两种红细胞的结果无统计学显著差异。
研究结果表明，MN表型不同的人类O型红细胞在病毒血凝滴定、再滴定和抑制反应中的反应速度和受体能力方面反应性相同，并且还表明与不含M和N血型抗原的木瓜蛋白酶处理红细胞相比，它们会出现病毒血凝现象，这间接意味着M和N血型抗原含有流感病毒的受体。
MM和NN表型的人类红细胞会与甲型流感病毒A2新加坡株出现病毒血凝现象，可用于常规病毒学诊断程序。O型血红细胞（MM和NN）在病毒血凝反应中，就反应速度和受体能力而言结果相同，并且对该反应结果有相同影响。经木瓜蛋白酶处理的红细胞不会引起病毒血凝现象，因为它们在红细胞膜表面不含病毒血凝素的受体，这些受体已被木瓜蛋白酶水解。红细胞膜表面的流感病毒受体是M和N血型抗原的组成部分，而这些抗原被木瓜蛋白酶破坏。
