Hillier A, Kwochka K W, Pinchbeck L R
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210, USA.
J Am Vet Med Assoc. 2000 Aug 15;217(4):536-40. doi: 10.2460/javma.2000.217.536.
To compare reactivities to intradermal injection of extracts of Dermatophagoides farinae, Dermatophagoides pteronyssinus, house dust mite mix, and house dust in dogs suspected to have atopic dermatitis.
Retrospective study.
115 dogs.
Records of all dogs suspected to have atopic dermatitis that underwent intradermal testing between October 1996 and July 1998 were reviewed. Reactivities to intradermal injection of crude mixed house dust mite (1:25,000 wt/vol) and crude house dust (25 PNU/ml) extracts were compared with reactivities to intradermal injection of individual extracts of D farinae and D pteronyssinus (1:50,000 wt/vol).
Ninety dogs were confirmed to have atopic dermatitis including 61 of the 69 dogs with positive reactions to either or both of the individual house dust mite extracts. Intradermal testing with the mixed house dust mite extract had sensitivity of 75%, specificity of 96%, and accuracy of 83%. Intradermal testing with the house dust extract had sensitivity of 30%, specificity of 93%, and accuracy of 56%.
Results suggest that use of crude mixed house dust mite and crude house dust extracts for intradermal testing in dogs is not as accurate a method of determining house dust mite hypersensitivity as is the use of individual D farinae and D pteronyssinus extracts mainly because of the high percentage of false-negative results. Extracts of individual house dust mites are recommended for intradermal testing of dogs suspected to have atopic dermatitis.
比较疑似患有特应性皮炎的犬只对皮内注射粉尘螨提取物、屋尘螨提取物、屋尘螨混合提取物及屋尘提取物的反应性。
回顾性研究。
115只犬。
回顾1996年10月至1998年7月间所有疑似患有特应性皮炎且接受皮内试验的犬只记录。将皮内注射粗制屋尘螨混合提取物（1:25,000重量/体积）和粗制屋尘提取物（25 PNU/ml）的反应性与皮内注射粉尘螨和屋尘螨单一提取物（1:50,000重量/体积）的反应性进行比较。
90只犬被确诊患有特应性皮炎，其中69只犬对一种或两种单一屋尘螨提取物呈阳性反应，61只在此之列。使用屋尘螨混合提取物进行皮内试验，敏感性为75%，特异性为96%，准确性为83%。使用屋尘提取物进行皮内试验，敏感性为30%，特异性为93%，准确性为56%。
结果表明，对于犬只，使用粗制屋尘螨混合提取物和粗制屋尘提取物进行皮内试验，在确定屋尘螨超敏反应方面不如使用粉尘螨和屋尘螨单一提取物准确，主要原因是假阴性结果比例较高。对于疑似患有特应性皮炎的犬只，推荐使用单一屋尘螨提取物进行皮内试验。
