Noli C, Bernadina W E, Willemse T
Department of Clinical Sciences of Companion Animals, University of Utrecht, Netherlands.
Vet Immunol Immunopathol. 1996 Jul;52(3):147-57. doi: 10.1016/0165-2427(96)05550-x.
The significance of reactions to crude extracts and purified fractions of the house dust mites Dermatophagoides pteronyssinus (Der p I and Der p II) and Dermatophagoides farinae (Der f I and Der f II) was evaluated in dogs with clinical manifestations of atopic dermatitis (AD). In 13 healthy control dogs and eight dogs with AD, immediate skin test reactivity was determined to serial dilutions of Der p I, Der p II, Der f I and Der f II. In addition, allergen-specific IgGd antibodies were determined by means of an enzyme-linked immunosorbent assay (ELISA) and Western blots. The results suggest that, in contrast to what occurs in humans and despite immediate skin test reactivity in some dogs, Der p I, Der p II, Der f I and Der f II are unlikely to be major allergens in dogs with AD. However, only serum of atopic dogs consistently binds a 90 kDa polypeptide of D. farinae, as shown by Western blot analysis.
在患有特应性皮炎（AD）临床表现的犬只中，评估了对屋尘螨（粉尘螨（Der p I和Der p II）和美洲大蠊（Der f I和Der f II））的粗提物和纯化组分的反应的意义。在13只健康对照犬和8只患有AD的犬中，对Der p I、Der p II、Der f I和Der f II的系列稀释液测定即时皮肤试验反应性。此外，通过酶联免疫吸附测定（ELISA）和蛋白质印迹法测定变应原特异性IgGd抗体。结果表明，与人类情况相反，尽管一些犬有即时皮肤试验反应性，但Der p I、Der p II、Der f I和Der f II不太可能是患有AD的犬的主要变应原。然而，如蛋白质印迹分析所示，只有特应性犬的血清始终与美洲大蠊的一种90 kDa多肽结合。
