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通过不连续白蛋白密度梯度离心分离的胸腺细胞增强骨髓生长。

Augmentation of marrow growth by thymocytes separated by discontinuous albumin density-gradient centrifugation.

作者信息

Pritchard L L, Shinpock S G, Goodman J W

出版信息

Exp Hematol. 1975 Apr;3(2):94-100.

PMID:1095384
Abstract

Mouse thymocytes were separated by discontinuous albumin density-gradient centrifugation. The ability of B6 thymocytes from the gradient fractions to increase the number of spleen nodules formed by B6 marrow cells in B6D2F1 recipients was compared with that of similar numbers of B6 thymocytes from an unfractionated cell suspension. Thymocytes from any of the gradient fractions were not more effective than thymocytes from an unfractionated suspension in augmenting marrow-cell growth, indicating that the presumptive 'effective cell type' in this system cannot be separated from the total thymocyte population by its buoyant density properties.

摘要

通过不连续白蛋白密度梯度离心法分离小鼠胸腺细胞。将来自梯度级分的B6胸腺细胞增加B6D2F1受体中B6骨髓细胞形成的脾结节数量的能力,与来自未分级细胞悬液的相同数量的B6胸腺细胞的能力进行比较。在促进骨髓细胞生长方面,来自任何梯度级分的胸腺细胞并不比来自未分级悬液的胸腺细胞更有效,这表明该系统中假定的“有效细胞类型”不能通过其浮力密度特性与整个胸腺细胞群体分离。

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