Watanakunakorn C, Glotzbecker C
Infect Immun. 1975 Jun;11(6):1182-6. doi: 10.1128/iai.11.6.1182-1186.1975.
The in vivo behavior of revertants of Staphylococcus aureus L-phase variants were studied in mice. The Giorgio strain of S. aureus was induced into L-phase variants by methicillin. After 13 serial passages in methicillin agar and six passages in antibiotic-free agar, reversion occurred. Six revertants, all phage untypable, coagulase negative, and mannitol negative, were inoculated intravenously into Swiss albino mice in comparison with the parent strain. Inoculation of 10(8) colony-forming units resulted in 38% mortality at 28 days for the Giorgio group and 0 to 2% for the revertants. The geometric mean titer of staphylococci in the kidneys was 3.5 x 10(5) for the Giorgio group and 0.5 x 10(0) to 1.0 x 10(2) for the revertants. Serial colony counts of blood, lungs, spleen, liver, and kidneys in mice inoculated with 10(7) colony-forming units showed faster clearance and failure of the revertant to colonize the kidneys. It is concluded that the in vivo behavior of the L-phase revertants was vastly different from that of the parent Giorgio strain.
在小鼠体内研究了金黄色葡萄球菌L型变异体回复株的行为。用甲氧西林将金黄色葡萄球菌的乔治亚菌株诱导为L型变异体。在甲氧西林琼脂中连续传代13次，在无抗生素琼脂中传代6次后，发生了回复。将6株回复株（均不可用噬菌体分型、凝固酶阴性且甘露醇阴性）与亲本菌株相比，静脉接种到瑞士白化小鼠体内。接种10⁸个菌落形成单位后，乔治亚组在28天时死亡率为38%，回复株组为0%至2%。乔治亚组肾脏中葡萄球菌的几何平均滴度为3.5×10⁵，回复株组为0.5×10⁰至1.0×10²。对接种10⁷个菌落形成单位的小鼠的血液、肺、脾、肝和肾进行连续菌落计数，结果显示回复株清除速度更快且未能在肾脏中定殖。得出的结论是，L型回复株的体内行为与亲本乔治亚菌株有很大不同。
