Qin Y, Wang C C, Kuhn H, Rathmann J, Pang C P, Rogers M S
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong.
BJOG. 2000 Aug;107(8):973-81. doi: 10.1111/j.1471-0528.2000.tb10399.x.
To determine the concentration of 8-isoPGF(2alpha) in cord blood as a measure of oxidative stress during labour, and to compare them with other established parameters of in vivo lipid peroxidation and with the acid-base status of the newborn.
Umbilical cord arterial and venous blood samples were collected from 81 singleton term deliveries for determination of 8-isoPGF(2alpha), malondialdehyde and organic hydroperoxides. In addition, metabolites derived from the oxidative metabolism of purines during hypoxia-reoxygenation and routine cord blood of oxygen saturation, pH, pO2, pCO2, HCO3 and base excess were measured.
Arterial concentrations of 8-isoPGF(2alpha) were significantly higher in cases with fetal distress, tight nuchal cord (P < 0.001), the umbilical coiling index, and male sex (P < 0.05) (R2 = 0.48). No correlation was found with any parameter of acid-base status. In arterial and venous blood the concentrations of organic hydroperoxides and hypoxanthine significantly correlated with the fetal nuchal cord (P < 0.001) (R2 = 0.26 and 0.16, respectively).
Our findings indicate that 8-isoPGF2(alpha) in cord arterial blood is a suitable parameter to quantify a possible oxidative stress in the fetus during labour. Measurements of the F2-isoprostane concentrations in cord blood at labour provide a clinically useful method to assess the perinatal outcome.
测定脐血中8-异前列腺素F2α（8-isoPGF(2α)）的浓度，作为分娩期间氧化应激的指标，并将其与体内脂质过氧化的其他既定参数以及新生儿的酸碱状态进行比较。
收集81例单胎足月分娩的脐动脉和脐静脉血样本，用于测定8-isoPGF(2α)、丙二醛和有机氢过氧化物。此外，还测量了缺氧-复氧过程中嘌呤氧化代谢产生的代谢产物以及常规脐血的氧饱和度、pH值、氧分压、二氧化碳分压、碳酸氢根和碱剩余。
胎儿窘迫、脐带绕颈紧（P < 0.001）、脐带螺旋指数以及男性胎儿（P < 0.05）（R2 = 0.48）的动脉血中8-isoPGF(2α)浓度显著升高。未发现与任何酸碱状态参数相关。在动脉血和静脉血中，有机氢过氧化物和次黄嘌呤的浓度与胎儿脐带绕颈显著相关（P < 0.001）（R2分别为0.26和0.16）。
我们的研究结果表明，脐动脉血中的8-isoPGF2(α)是量化分娩期间胎儿可能存在的氧化应激的合适指标。分娩时测定脐血中F2-异前列腺素浓度为评估围产期结局提供了一种临床有用的方法。
