Mok H Y, von Bergmann K, Grundy S M
J Lipid Res. 1979 Mar;20(3):389-98.
Hepatic outputs of biliary lipids can be measured by intestinal perfusion techniques, either during constant infusion of liquid formula into the duodenum or through-out a 24-hour period during which time three meals are given along with an overnight fast. The purpose of this study was to compare these two methods for estimating secretion of biliary lipids. In 21 subjects, mean hourly outputs measured during continuous feeding were highly comparable to those during intermittent feeding, showing that the constant infusion technique gave valid estimations of overall daily secretion rates of biliary lipids. On the other hand, the intermittent mode of feeding showed phasic changes of outputs and composition of biliary lipids in response to feeding and fasting apart from total outputs over 24 hours. However, it takes longer to complete and requires the use of a meal marker. By combining the intestinal perfusion technique with measurements of fecal excretion of neutral steroids and bile acids, this method may be used to estimate absorption of cholesterol and bile acids from the intestine. Thus, these measurements allow quantification of a number of parameters of the enterohepatic circulation.
胆汁脂质的肝脏输出量可通过肠道灌注技术来测量，既可以在向十二指肠持续输注液体配方饮食期间进行测量，也可以在24小时内进行测量，在此期间提供三餐并禁食一夜。本研究的目的是比较这两种估计胆汁脂质分泌的方法。在21名受试者中，持续喂养期间测得的平均每小时输出量与间歇喂养期间的输出量高度可比，这表明持续输注技术能够有效估计胆汁脂质的总体日分泌率。另一方面，除了24小时的总输出量外，间歇喂养模式显示胆汁脂质的输出量和成分会随着进食和禁食而发生阶段性变化。然而，这种方法完成起来需要更长时间，并且需要使用膳食标记物。通过将肠道灌注技术与中性类固醇和胆汁酸的粪便排泄量测量相结合，该方法可用于估计肠道对胆固醇和胆汁酸的吸收。因此，这些测量能够对肠肝循环的多个参数进行量化。
